《植物生理学报》 2017, 53(8): 1409-1418

NaCl胁迫对19个苦荞品种生理特性及FtNHX1表达的影响

陆启环¹, 李发良², 张弢¹, 于延冲¹, 杨德翠¹, 高婷¹, 侯晓敏¹, 董春海¹, 杨洪兵^1,*

¹青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109; ²四川省凉山州农业科学研究所高山作物研究站, 四川凉山616150

通信作者：杨洪兵；E-mail: hbyang@qau.edu.cn

摘　要：

以‘晋荞2号’、‘迪苦1号’、‘西农9909’、‘西农9920’、‘西农9940’、‘西农9943’、‘黔苦2号’、‘黔苦3号’、‘黔苦4号’、‘黔苦5号’、‘黔苦7号’、‘昭苦1号’、‘‘昭苦2号’、‘‘西荞2号’、‘西荞3号’、‘云荞1号’、‘云荞2号’、‘九江苦荞’和‘榆6-21’等19个苦荞品种为实验材料, NaCl胁迫下测定相关耐盐生理指标, 并对耐盐基因FtNHX1表达量进行分析, 以筛选耐盐和盐敏感苦荞品种。结果表明, NaCl胁迫下‘黔苦3号’种子发芽率和幼苗SOD活性降低幅度较小, 幼苗质膜透性和MDA含量增加幅度较小, 说明盐胁迫下‘黔苦3号’生理特性较为稳定, 且耐盐基因FtNHX1在主要拒Na⁺部位表达量的增加幅度最大, 可以有效减少盐分向地上部运输; 而‘西农9909’幼苗质膜透性和MDA含量增加幅度较大, 说明盐胁迫下其膜系统受到很大伤害, 且幼苗叶绿素含量和SOD活性降低幅度较大。因此, 19个苦荞品种中‘黔苦3号’的耐盐性最强, 是耐盐品种; ‘西农9909’的耐盐性最差, 是盐敏感品种。

关键词：苦荞; 盐胁迫; 生理特性; FtNHX1表达量; 耐盐品种; 盐敏感品种

收稿：2017-03-25   修定：2017-07-05

资助：国家自然科学基金(31371552)。

Effect of NaCl stress on physiological characteristics and FtNHX1 expression of nineteen tartary buckwheat varieties

LU Qi-Huan¹, LI Fa-Liang², ZHANG Tao¹, YU Yan-Chong¹, YANG De-Cui¹, GAO Ting¹, HOU Xiao-Min¹, DONG Chun-Hai¹, YANG Hong-Bing^1,*

¹Key Laboratory of Plant Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong 266109, China; ²Research Station of Alpine Crop, Department of Agriculture and Science, Liangshan, Sichuan 616150, China

Corresponding author: YANG Hong-Bing; E-mail: hbyang@qau.edu.cn

Abstract:

The nineteen tartary buckwheat varieties including ‘Jinqiao No.2’, ‘Diku No.1’, ‘Xinong 9909’, ‘Xinong 9920’, ‘Xinong 9940’, ‘Xinong 9943’, ‘Qianku No.2’, ‘Qianku No.3’, ‘Qianku No.4’, ‘Qianku No.5’, ‘Qianku No.7’, ‘Shaoku No.1’, ‘Shaoku No.2’, ‘Xiqiao No.2’, ‘Xiqiao No.3, ‘Yunqiao No.1’, ‘Yunqiao No.2’, ‘Jiujiang tartary buckwheat’ and ‘Yu 6-21’ were used as the experimental materials. Though determining the physiological indexes of salt tolerance and analyzing the expressions of salt tolerance gene FtNHX1 under NaCl stress to select the salt tolerant and salt sensitive tartary buckwheat variety. The results showed that the seeds germination rate and seedlings SOD activity of ‘Qianku No.3’ were decreased slightly under NaCl stress, and the seedlings plasmalemma permeability and MDA content were increased slightly. These indicated that the physiological characteristics of ‘Qianku No.3’ were smoothly changeding under salt stress, and the expression of FtNHX1 in the main Na⁺ exclusion localization increased obviously, which could effectively prevent the salt transport to shoot. While the seedlings plasmalemma permeability and MDA content of ‘Xinong 9909’ were increased greatly, indicating the membrane system was harmed greatly under salt stress, and the seedlings chlorophyll content and SOD activity were decreased significantly. Therefore, ‘Qianku No.3’ was the most salt tolerant one among the nineteen tartary buckwheat varieties, which was a salt-tolerant variety, while ‘Xinong 9909’ was a salt-sensitive variety.

Key words: tartary buckwheat (Fagopyrum tataricum); salt stress; physiological characteristics; relative expression of FtNHX1; salt tolerant variety; salt sensitive variety